For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQiagen omniscript rt kit Omniscript Rt Kit, supplied by Qiagen, used in various techniques. Omniscript Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in E. The ideal reverse transcriptase is robust (highly active under a variety of conditions) and converts all primed RNA within a sample to cDNA, regardless of its abundance, length or secondary structure. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. OmniScripts allow you to craft dynamic customer. Quantitative RT-PCR Analyses Total RNA of the collected samples were extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. This kit is ideal for analyzing low input RNA samples. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. High Ca2+ stimulated the upregulation (as assessed by RT-PCR) and the secretion of Wnt5a (assessed by Western blot), a. It allows for the efficient labeling of a wide range of starting RNA concentrations and. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR(RT-PCR) RNA was extracted from cultured macrophages with RNeasy Mini Kit (Qiagen, Hilden, Germany) and made into cDNA with Omniscript RT Kit (Qiagen) according to the manufac-turer’s instructions. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Format. At this reaction temperature, common restrictions of RT-PCR are removed, simplifying assay design and eliminating compromises. nos. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. The OneStep Ahead RT Mix contains an RNase inhibitor and a blend of Omniscript® and Sensiscript® reverse transcriptases in combination with an RT-blocker for heat-mediated activation of the reverse-transcription step. 1 ml 4. Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. ·. Omniscript RT system, whereas substantial copies of. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. However, RT is recognized as a known source of variability, particularly with low amounts of RNA. RT-qPCR was carried out inRNA concentration was photometrically determined, and 200 ng RNA was reverse transcribed using Omniscript RT kit with oligo dT Primer (Qiagen, Hilden, Germany). For standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. QIAstock. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 在终点法PCR中，用于RNA模板量为50 ng到2 μg的逆转录反应For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR高感度リアルタイム2ステップRT-PCRによる遺伝子発現解析 でcDNA合成とgDNA除去をわずか20分で実現する高速 cDNA合成のために、 5'および3'の転写産物の少ないcDNA合成でも高いcDNA収量 を実現。 RNA特異的なプライマーやプローブ ユニークなQuantiTect逆転写キットは. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g RNA/Reaction for end-point PCR, Includes 200U Omniscript Reverse. The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. The one-step format prevents contamination and allows for higher sensitivity. reverse transcription, and quantitative PCR to work optimally in archived clinical samples. Reverse Transcription Kit (Omniscript® RT Kit) as per the manufacturer's instructions (Qiagen). The maximum amount of viral RNA was used in place of water for both reactions. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. This product is not intended for the diagnosis, prevention, or treatment of a disease. Both. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Balanced mixture of enzymes with optimized reverse-transcription buffer The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of “difficult” (e. 1 ml RNase-free water 205111 询价The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. icon_0011_idea-s. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. , Valencia, CA). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. # 18064 and 18080, respectively), and Omniscript® and Sensiscript® (Qiagen Cat. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. cDNA was generated with the help of the Omniscript RT Kit (Qiagen), and a forward primer that overlaps the boundaries of exon 8 and exon 9, HNRNPU-forward 5′. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. USD $1207. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. 31 Australia QIAGEN Pty Ltd PO Box 641 DONCASTER VIC 3108 Orders 03-9840-9800 Fax 03-9840-9888 Technical 1-800-243-066 Belgium QIAGEN Benelux B. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. The sequences of the primers used in. DSL Modem has an attribute Grade (ATT_RT_GR) and possible values are Good, Better and Best. An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. File size. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Omniscript RT Kit. Reactions were incubated at 65°C for 5 min, then 100 U RT enzyme was added and incubated at 37°C for 60 min and 75°C for 5 min. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the step of PCR amplification. Background Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. For example, performance of OmniScript and SensiScript (both Qiagen) or AMV-derived RTases have been heavily influenced by experimental. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. The reverse transcription (RT) step conditions for all primer sets were 30 min at 50 C and 15 min at 95 C. It is crucial to recognize and prevent potential issues with cDNA synthesis in order to maintain the reliability of experimental. First-strand cDNA was synthesized from 1 μg of high-quality total RNA using SuperScript II Reverse Transcriptase in accordance with the manufacturer’s instructions (QIAGEN, Omniscript RT). To understand the role of the colonic extracellular calcium-sensing receptor (CaSR) in calcium chemoprotection against colon cancer, we activated the CaSR with 5 mM Ca2+ on HT-29 cells, an adenocarcinoma cell line. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. The enzyme mix is specially formulated for both reverse transcription and PCR. CNV qPCR Assays & Arrays;. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. Qiagen omniscript reverse transcription rt kit Omniscript Reverse Transcription Rt Kit, supplied by Qiagen, used in various techniques. During reverse transcription, chemically modified HotStarTaq DNA Polymerase is completely inactive and does not interfere with the reverse-transcriptase reaction. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. RT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. For reverse transcription from 50 ng to 2 μg RNA per reaction for end-point PCRAdditional Information. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For reverse transcription of 50 ng to 2 μg RNA per reactivity for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROverall, my experience with Qiagen’s OmniScript RT Kit has been good. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. 1 ml 4. For each RTase, the reaction conditions and thermal profile followed the recommended protocol issued by the manufacturer (see Protocols in online Data Supplement 1). ). Serial analysis of BCR-ABL mRNA levels by real-time quantitative PCR (RQ-PCR) accurately reflects the level of leukemic inhibition induced by therapy and provides. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription is described based on the Omniscript ® Reverse Transcription Kit (Qiagen). OmniScript を使用しましょう。. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR OmniScripts. Omniscript is designed for reverse transcription of RNA amounts greater than 50 ng, andFor reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRExperimental validation. 5 mM each dNTP, 10 U of RNase inhibitor, 4 U of Omniscript Reverse Transcriptase, and. 1. Marketplace Solutions. Sensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. All seven samples were reverse transcribed by the same kit toFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. We compared these five RTs for their utility in detecting trace transcripts by real-time PCR, ability to transcribe a long RNA and sensitivity. Omniscript, SuperScript III and PowerScript (P < 0. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Need Assistance?. CNV qPCR Assays & Arrays;. Product Description. 2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR. Taqman primers of selectin E. Omniscript Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. , the OmniScript RT, an RNAse H+ RT, which is part of the QuantiTect Reverse Transcription Kit, as well as with RNase H. PCR was performed with HotStarTaq PCR Kit (Qiagen). EGFP cDNA were obtained with th e other RTases. QIAsphere Digital Connectivity. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 × 150 µL 10 × Buffer RT, 4 × 100 µL dNTP Mix (contains 5 mM each dNTP), 4 × 1. SYBR Green (DyNAmo SYBR Green qPCR Kit; Finnzymes, Finland) and related accessories to perform real-time RT-qPCR (Opticon2; MJ Research, Watertown,. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. The cDNA was generated using the Omniscript RT kit (Qiagen) with random hexamers (Roche Products, United States). A high affinity for RNA allows Omniscript Reverse. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. CNV qPCR Assays & Arrays;. USD $1207. The cDNA was then synthesized with an Omniscript Reverse Transcription (RT) Kit (Qiagen) or Moloney Murine Leukemia Virus Reverse Transcriptase Reverse Transcriptase (M-MLV RT). 1 mL RNase-free. Bioz Stars score: 86/100, based on 1 PubMed citations. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. OmniStudio Standard. Subsequent PCR was performed with primers (Sigma-Aldrich) based on the Icelandic rock ptarmigan genome and Taq DNA polymerase (QIAGEN). 205110, 205111and 205113) should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer. アフィニティーが高い酵素による高収量cDNA わずか10コピーのテンプレートも高感度で検出 追加のRNase H分解は不要 煩わしいピペッティング操作なしの迅速で簡単な製法 Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ng. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. Add 2 μl of Buffer RT (component of Omniscript RT Kit), 2 μl of dNTP Mix (component of. Matrix assay. Nine parallel large-scale GoScript™ RT reactions were performed, containing log-fold variations of input human reference total RNA (10ng/µl to 0. Short cycling steps withoutExplore targets and avenues in their scientific context, find and adjusting products till study i, analyze details and plan follow-up studies – all in GeneGlobe. For reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCR Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ngから2 µgのRNA量を用いた逆転写用に特別にデザインされています。 RNAへのアフィニティーが高いOmniscript Reverse Transcriptaseは、他の逆転写酵素と比較して非常に優れた性能を示し、低いコピー数の. This kit's protocol is simple and suitable for high throughput. Product tracking (in bp) are shown about the right of the panel (lane 9). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor backward transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Semiquantitative RT-PCR. There are several commercially available RT kits which differ mainly in the kind of enzyme they use, bacterial (rTth) or viral origin (e. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. Qiagen confirmed that such a plasmid is. (2010). A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Total RNA from flag leaves of mature rice plants was isolated using the RNeasy Plant Mini Kit (Qiagen, Venlo, The Netherlands) and reversely transcribed using Omniscript RT Kit (Qiagen). g. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. 0 µl of Omniscript or Sensiscript RT (Qiagen, Santa Clarita, CA), 2. アフィニティーが高い酵素による高収量cDNA わずか10コピーのテンプレートも高感度で検出 追加のRNase H分解は不要 煩わしいピペッティング操作なしの迅速で簡単な製法 Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ng. Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. PCR was performed on an ABI 7900HT using TaqMan® Universal PCR Master Mix, No Amperase® UNG (Life Technologies, Carlsbad, CA) or. OmniScripts are built by moving elements into the canvas and defining their properties. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. Place a nuclease-free PCR tube on ice and add approximately 2 μg of isolated total RNA. 1 ml 4. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReal-time RT-PCR A OneStep RT-PCR Kitb was used with a 25-#l reaction volume. One microliter of kit-supplied enzyme mix-ture (combination of Omniscript Reverse. 2) If you only wanted to produce cDNA from a subset of all mRNA, then. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. English. Life Science Grants. Omniscript RT Kit. Omniscript and Sensiscript QuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. 0 µl 5 mM dNTP (final concentration of 500 µM), 2. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. QIAGEN Subsidiaries. Additional Information. $1,382. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. . For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRBalanced mixture of enzymes with optimized reverse-transcription buffer The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of “difficult” (e. show that ferrets are highly susceptible to SARS-CoV-2 infection and effectively transmit the virus by direct or indirect contact, recapitulating human infection. Please see the Omniscript Reverse Transcription Handbook for protocols. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. OmniStudio. , SuperScript vs. , Valencia, CA). g. Differences Between OmniStudio and OmniStudio for Vlocity. 1 ml 1. CNV qPCR Assays & Arrays;. Global Contacts. Reverse Transcription Troubleshooting Guide. A5003), SuperScript® II and SuperScript® III (Invitrogen Cat. OmniScript を使用しましょう。. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Workflow for Configuring OmniAnalytics for a Third-Party Analytics. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. 1). Omniscript is designed for reverseFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThis study provides a complex overview of the influence of elements such as RT systems, qRTPCR chemistry, amount of background RNA, and transcript abundance on the efficiency of qRT-PCR. Mix carefully by vortexing for no more than 5 s, and centrifuge briefly to collect residual liquid from the sides of the tube. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 x 150 µl 10x Buffer RT, 4 x 100 µl dNTP Mix (contains 5 mM each dNTP), 4 x 1. PCR and RT-PCR are achieved by the use of the hot-start enzyme HotStarTaq Plus DNA Polymerase together with a specialized fast PCR or RT-PCR buffer. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. Amplification and detection were performed with the ABI7500 Sequence Detection System (Applied Biosystems, Foster City, CA). 1 ml 4. Reverse transcription reaction conditions RT kits used in this experiment were chosen to represent the most commonly used enzymes in the field (Fig. The easy one-tube setup and optimized components enable higher sensitivity. ZERO BIAS - scores, article reviews, protocol conditions and moreFor 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. CNV qPCR Assays & Arrays; Microbial DNA qPCR Assays & Panels For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Qiagen omniscript rt kit Omniscript Rt Kit, supplied by Qiagen, used in various techniques. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Differences Between OmniStudio and OmniStudio for Vlocity. 1 ml 1. Your reverse transcription may not be optimal. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. CNV qPCR Assays & Arrays;. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Please see the Omniscript Reverse Transcription Handbook for protocols. A PCR was conducted using standard PCR methods (primers are available on request). OmniScript は、お客様がビジネスプロセスを完了するためのガイド付きパスを提供し、シームレスなカスタマーエクスペリエンス (これは 常に 好ましいことです) を実現するため. The purification steps can be performed as described in the protocol using Qiagen’s PCR purification kit. In order to measure this difference on a large spectrum of background RNA, given that DNA and RNA concentrations are important influencing factors [ 8 - 10 ], the study used total RNA extracted from the same testis tissue (Methods). My QIAGEN. In a final. This kit's protocol is simple and suitable for high throughput. The QIAGEN One-Step Ahead RT-PCR Kit contains a blend of Sensiscript and Omniscript Reverse Transcriptases, a well-balanced combination of Taq DNA Polymerases and a proofreading enzyme. This kit is ideal for analyzing low input RNA samples. RNA was quantified using a nanodrop spectrophotometer (Thermo) and reverse transcribed to cDNA with the Omniscript RT Kit (Qiagen, 205110) as per the manufacturer's instructions. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. 2800. AIV genomes were amplified from extracted RNA using universal and gene-specific primers with an Omniscript reverse-transcription kit (QIAGEN, Germantown,. DSL Service has an attribute Download Spee d (ATT_RT_DS) and possible values are 7, 12, 20 and 40 Mbps. Actin and CD30 cDNA transcripts were quantified using AB&I TaqMan primer and probe sets (Life Technologies) on a Bio-Rad CFX96 real-time. 1 ml RNase-free water 205111 询价 The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. 00. Two micrograms of total RNA was reverse-transcribed using Omniscript RT kit (Qiagen). The PCR products were resolved by electrophoresis on 1% agarose gels and visualized by ethidium bromide staining. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). 1 ml 4 x. . For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Reverse Transcription Kit Quick-Start Protocol (EN) Print Bookmark Share pdf 56KB English Format File size Language Download Get Adobe Reader Contact QIAGEN . 1 ml RNase-free water 205111 询价 Reverse transcription and quantitative real-time PCR (RT-qPCR). The QIAGEN OneStep RT-PCR Enzyme Mix contains a specially formulated enzyme blend for both reverse transcription and PCR. Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. この例には、再利用可能な. The ideal reverse transcriptase is robust (highly active under a variety of conditions) and converts all primed RNA within a sample to cDNA, regardless of its abundance, length or secondary structure. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. g. First-strand cDNA synthesis was done using the Omniscript RT kit (Qiagen, Valencia, CA) as recommended by the manufacturer. coli. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription from 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). 10µl fractions of each reverse transcription reaction product, representing 100ng to 1fg of. Spoorstraat 50 5911 KJ Venlo The Netherlands Orders 0800-79612 Fax 0800-79611 Technical 0800-79556 Canada QIAGEN Inc. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe reverse transcription was achieved using Omniscript RT Kit (Qiagen), according to manufacturer’s instructions. 0 µl of 10 µM H-T11C (final concentration of. . Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. 05). The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. RNA, using the Omniscript RT kit (Qiagen Inc. Content. 1 ml 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRRT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. RT-PCR assays were performed as previously described (Rosciszewski et al. The ABScript II Reverse Transcriptase in the. Please see the Omniscript Reverse Transcription Handbook for protocols. The rela‑ tive gene expression levels were normalized to GAPDH and calculated using the 2‑ΔΔCq method. 3. Related products . An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-free water 1. Sensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript®andSensiscript®RT WiderangeofRNAamounts(1pg–2µg) Highsensitivity OneStepRT-PCRBuffer Minimaloptimizationneeded. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Technical Service; Customer Care . Vlocity Cards Framework. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript® Reverse TranscriptionFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR3 min read. 00. High yields of cDNA 특이성을 높인 참신한 enzyme과 buffer No additional RNase H digestion step required Robust system guarantees consistent results The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. However, the Sensiscript and PowerScript systems were more efficient for detecting high. 0 μg of RNA was reverse-transcribed using the Omniscript RT Kit (Qiagen Inc. After the cDNA was diluted 1:3 in nuclease-free water, qRT-PCR was performed using the custom-designed primers (Biomers, Ulm, Germany) in Table 1 and the Platinum SYBR Green qPCR SuperMix-UDG kit (Invitrogen), or TaqMan Gene Expression Assays and. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. This also inactivates the reverse transcriptases. Unit definition: 2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR Discover the products and protocols you need Plan your entire experiment based on research interests. これにより、特定の取引先責任者や取引先の項目を表示するなどの一般的な機能を持つ小さな「子」 OmniScript を作成することができます。. Omniscript is a confident hand-lettered script without self-conscious style or idiosyncrasy. Four weights together with monospaced numerals and math symbols make it ideal for. After reverse transcription by Omniscript and Sensiscript Reverse Transcriptases, reactions are heated to 95°C for 15 minutes to activate HotStarTaq DNA Polymerase and toFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. These elements are placed in a specific sequence for the workflow. Pharmaceutical Industry. For the reverse-transcription step, both Omniscript and Sensiscript are functionalized with an RT-blocker, which keeps them in an inactive state and prevents nonspecific transcription at ambient temperature. Total RNA was isolated from drug-treated cells with TRIzol (Invitrogen) and then reverse-transcribed to cDNA using the Omniscript RT kit (Qiagen, Valencia, CA). The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. RT-PCR Assays. 1fg/µl). A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR. 1 ml RNase-free water. OmniScripts 要素 (子 OmniScript) は、OmniScript をコールして別の OmniScript 内で再利用します。. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. There are elements for: User. Yet, the RT reactions for standard curves constructed using Sensiscript and Omniscript produced reliable Cts at target concentrations as low as 10 3 copies/μl, similar to that observed for SSIV and SSIII (except for Sensi-RH: lower limit at 10 4 copies/μl). DSL Modem Product. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The ABScript II Reverse Transcriptase in. 1 ml 4. 1 ml 1. using an Omniscript reverse transcriptase kit (Qiagen, Valencia, CA) in a 20- l reaction mixture, containing 200 ng of total RNA and specific primers, at 37°C for 1 h. SuperScript II, AMV-RT, Transcriptor RT, M-MuLV RT, M-MLV RT, Omniscript, DyNAmo, Statascript: Covariances of the RT efficiency were driven by target gene or total RNA concentration: Enzyme differences less important than diversity in gene-specific RT reproducibility: Bustin et al. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Migrate to Standard OmniStudio. IL-10 was demonstrated to be critical for suppressing autoimmunity and inflammatory responses ( 40 – 43 ), and whether Th17 express IL-10 is. Kit contents: Qiagen Omniscript RT Kit, 200 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. QIAGEN now offers the first commercially available reverse transcriptases from a new source. Specific genes have been used as baseline controls to. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. Reverse transcription is a powerful tool for creating complementary DNA (cDNA) from RNA, which can then be used in a variety of downstream applications for studying RNA. When the reaction is heated to 50°C, the RT-blocker dissociates from the RT enzymes, rendering them fully active. Data Migration with Build Tool. nos. Reverse transcription was applied with Omniscript RT Kit following manufacturer's protocol (Qiagen). Inquiry. All reactions took place for 1h at 37°C in a final volume of 40 μl containing 1 μg total RNA in the presence of 20 units RNase inhibitor (RNasin® Ribonuclease Inhibitor, Promega), 1 μg random hexamers (Microsynth), 4 μl 10. Bioz Stars score: 96/100, based on 1 PubMed citations. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Briefly, RNA was isolated using the RNAeasy Mini kit (Qiagen, Germany). # 205113 and 205213, respectively) reverse transcriptases. Reverse transcriptase is a multifunctional enzyme with three distinct enzymatic. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. First Strand kit)? What is recommended? We have had a good experience with both types of Reverse Transcriptases, e. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription was carried out according to the manufacturer's protocol either with the Omniscript RT (Qiagen) or SuperScript II RT (Invitrogen). RNA was the same set of samples used for Illumina GAII sequencing analysis. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniScripts. EGFP cDNA were obtained with th e other RTases. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. Investor Relations Contacts. RT-PCR; PCR for Difficult Targets; Fast PCR; Long PCR; Direct PCR; High Fidelity PCR; Hot Start PCR; Real-Time PCR; Routine PCR ReagentOmniscript Rt Kit, supplied by Qiagen, used in various techniques. 0 µl 10× RT buffer, 2. The Omniscript RT Kit is intended for molecular biology applications. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Enzyme activity: Reverse tran…The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50. IVDR Support. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFAQ. Key words: Breast cancer, Formalin-fixed, Paraffin-embedded tissue, Quantitative RT-PCR,Reverse Transcription was performed using an OmniScript™ RT kit (QIAGEN) according to manufacturer’s instructions and using gene-specific primers to interrogate genes of interest. g. 1 ml 1. RT-PCR Analysis . For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. 95°C after the reverse transcription step. Direct quantification of six different targets in human total RNA using GoTaq® 2-Step RT-qPCR System. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. A Vlocity OmniScript guides users through complex processes with fast, personalized, and consistent responses. less than 500 ng total RNA). Real-time PCR analysisReverse Transcriptase was performed using Omniscript RT kit (Qiagen, Valencia, CA, #205113) according to manufacturer’s protocol. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Bioz Stars score: 86/100, based on 1 PubMed citations. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. 1 ml 1. Both biological and technical triplicates were run. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich. PCR reactions were performed to determine Clk 1 mRNA levels using JumpStart Ready-Start amplification mix (Sigma) and previously published primers. It relies on the principle of reverse transcription which is a process ‘reverse’ to the transcription. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 47 / EA. The one-step format prevents contamination and allows for higher sensitivity. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Omniscript reverse transcriptase (QIAGEN) using random hexamers and oligo dT and. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). ,. Total RNA was reverse-transcribed to cDNA with the use of Qiagen Omniscript RT Kit at 37°C for 60 minutes. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. PCR primers were designed using online available primer design software from Thermo Fisher Scientific and were purchased from Thermo Fisher Scientific. Sample to Insight__ Quick-Start Protocol March 2016 Omniscript® Reverse Transcription Kit The Omniscript RT Kit (cat. The total volume per reaction is set to 20 μl. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. This enzyme combination provides highly efficient and sensitive reverse transcription over a wide range of RNA templateLightning Experience. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. OmniStudio.